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Accueil veterinaires comportementalistes Publications internationales veterinaires comportementalistes Effects of dog-appeasing pheromones on anxiety and fear in puppies during training and on long-term socialization

Effects of dog-appeasing pheromones on anxiety and fear in puppies during training and on long-term socialization

Sagi Denenberg, DVM; Gary M. Landsberg, DVM, DACVB

Revue : Journal of the American Veterinary Medical Association

North Toronto Animal Clinic, 99 Henderson Ave, Thornhill, ON L3T2K9, Canada (Denenberg, Landsberg)

Supported by a grant from CEVA Sante Animale, France.
Dog-appeasing pheromone and placebo collars were supplied by CEVA Sante Animale, France.



To evaluate the effectiveness of dog-appeasing pheromone (DAP) in reducing fear and anxiety in puppies and its effects on training and socialization.


Randomized, controlled clinical trial.


45 puppies between 12 to 15 weeks of age at the time of inclusion.


Puppies enrolled in puppy classes were randomly allocated to 1 of 4 groups: 2 large-breed groups (1 DAP and 1 placebo group) and 2 small-breed groups (1 DAP and 1 placebo group). The investigator, trainers, and owners were unaware of treatment allocation throughout the study. Classes lasted 8 weeks, and owners were asked to complete a questionnaire before the first lesson and at the end of each lesson thereafter. Data collected included amount of learning and degrees of fear and anxiety for each puppy. Follow-up telephone surveys of owners to obtain information on subsequent socialization of puppies were performed at 1, 3, 6, and 12 months after the classes ended.


Dogs in DAP and placebo groups were significantly different with respect to degrees of fear and anxiety; longer and more positive interactions between puppies, including play, were evident in dogs in the DAP groups. Data from follow-up telephone surveys indicated that puppies in the DAP groups were better socialized and adapted faster in new situations and environments, compared with puppies in the placebo groups.

Conclusions and Clinical Relevance

When compared with a placebo treatment, DAP was useful in reducing anxiety and fear in puppies during puppy classes and resulted in improved socialization.


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a. DAP, CEVA Sante Animale, Intervet/Schering-Plough Animal Health Canada Inc, Kirkland, QC, Canada.

b. SAS, version 8.2, SAS Institute Inc, Cary, NC.

Address correspondence to Dr. Denenberg.

publications scientifiques


JAVMA - December 15, 2008, Vol. 233, No. 12, Pages 1874-1882



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